ELISA measurement of LDL receptors

Measurement of protein carbonyls in plasma of smokers and in oxidized LDL by an ELISA.

Protein carbonyls are considered as markers of oxidative stress (1 ). The conventional colorimetric assay (2 ) that measures reactive protein carbonyls using 2,4-dinitrophenylhydrazine (DNPH) is labor-intensive, time-consuming, and uses a large quantity of solvents. Recently, a new ELISA method for protein carbonyls was developed (3 ). However, the validity of this assay in smokers, who clearly...

Measurement of transcobalamin by ELISA.

BACKGROUND Transcobalamin is essential for the cellular internalization of cobalamin. Methods to quantify the unsaturated protein are available, but few attempts have been made to develop methods to quantify the sum of unsaturated and cobalamin saturated transcobalamin. METHODS gamma-Globulins from two polyclonal rabbit antibodies against recombinant human transcobalamin were used as capture ...

Direct Measurement of LDL Cholesterol

able amounts of radiolabeled dextran in the plasma protein fraction [15]. Consequently, we do not think this mechanism plays a substantial role in producing the lower than expected dextran concentrations seen in the present study. The dextran we used had a mean molecular mass of 70 000 Da. Thus, some quantity of lower-molecular-mass dextran could have leaked out of the vascular space and distri...

Measurement of Epsilon Antitoxin in Sheep Sera by ELISA

Measurement of lymphokine receptors.

One of the most widely studied lymphokine systems is the T lymphocyte growth factor interleukin 2 (IL-2). This unit describes two basic methods for the quantitation and biochemical characterization of IL-2 receptors. The first method employs the radioreceptor assay. Support protocols to this technique describe quantification of data via calculations of association and dissociation rates and the...